Collect. Czech. Chem. Commun.
1984, 49, 1552-1556
https://doi.org/10.1135/cccc19841552
Effect of the hydrophilicity of the polymer matrix on immobilized α-chymotrypsin
Minoru Kumakura and Isso Kaetsu
Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Takasaki, Gunma, Japan
Abstract
α-Chymotrypsin was immobilized by radiation polymerization at low temperatures and the effect of the hydrophilicity of the polymer matrix on the enzyme activity and thermal stability was studied. The activity and thermal stability of immobilized chymotrypsin increased with the increasing hydrophilicity of the polymer matrix or monomer. The thermal stability was affected by the form and pore size of the polymer matrix; chymotrypsin immobilized on a soft-gel polymer matrix exhibited an enhanced thermal stability.
