Collect. Czech. Chem. Commun.
1990, 55, 2765-2768
https://doi.org/10.1135/cccc19902765
Fluorescence studies on P1,P3-dinucleoside triphosphates related to mRNA cap: Acidity and intramolecular stacking
Zbigniew Wieczoreka, Edward Darżynkiewiczb, Janusz Stępińskic and Harri Lönnbergd
a Department of Physics and Biophysics, Academy of Agriculture and Technology, 10-957 Olsztyn, Poland
b Department of Biophysics, University of Warsaw, 02-089 Warsaw, Poland
c Department of Chemistry, University of Warsaw, 02-093 Warsaw, Poland
d Department of Chemistry, University of Turku, SF-20500 Turku, Finland
Abstract
The fluorescence intensity of several P1,P3-dinucleoside triphosphates related to mRNA cap-structure has been measured as a function of pH and temperature. The extent of intramolecular stacking has been estimated by comparing the intensities obtained with dinucleoside triphosphates to those of the corresponding monomers. The pKa values of 7-alkylguanine moieties in both dimeric and monomeric compounds have been determined by fluorescence and absorption spectroscopy.
